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481.
G. Eisner V. Negruk A. Hannoufa B. Lemieux 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(5-6):801-809
A number of genes involved in the biosynthesis of the epicuticular wax (EW) of Arabidopsis thaliana have recently been isolated through genetic approaches. In view of the evidence in favor of the importance of EW compounds
in the adaptation of higher plants to a number of physiological and ecological stresses, we have used clones of some of these
genes to genetically engineer constructs with which to manipulate EW biosynthesis in transgenic A. thaliana plants. All our constructs were placed under the control of the near constitutive CaMV 35S promoter. We were able to complement
mutant plants with the construction in the sense orientation as well as induce phenocopies of the eceriferum phenotype by transforming wild-type plants with both the sense and antisense constructs. We observed reduced fertility in
the wild-type plants transformed with the 35SCER3sense or 35SCER3antisense constructs but not in those transformed with the
35SCER2sense or 35SCER2antisense constructs.
Received: 28 December 1997 / Accepted: 31 March 1998 相似文献
482.
Rick C. Heupel 《Phytochemistry》1985,24(12):2929-2937
The intraspecific similarities and differences among the various polycyclic isopentenoids (sterols and pentacyclic triterpenes) which occur in leaf tissue and surface wax from three varieties of flowering Sorghum bicolor; G499 GBR, BOK 8 and IS 809, have been determined. The three varieties exhibited differences in phenotypic characters (e.g. shoot height) and pest resistance. While sterol and pentacyclic amyroid compositions were similar in the three varieties, significant differences were evident in the qualitative distribution of the migrated hopanoids. One variety, IS 809, which is phenotypically short and resistant, contained a single migrated hopanoid, sorghumol Alternatively, the other two varieties, both phenotypically tall and one-G 499 GBR-resistant, the other-BOK 8-susceptible, contained a mixture of four Δ9(11)-migrated hopanoids, i.e. sorghumol and three of its stereoisomers, and the Δ5(6)-migrated hopanoid simiarenol. While ketones corresponding to the Δ9(11)-migrated hopanoids were detected in the three varieties, the ketone of the Δ5(6)-migrated hopanoid was apparently absent. In contrast to other graminaceous plants, the predominant C-3 derivatives did not include the C-3 methyl ethers, but did include esters and polar conjugates. Significant differences apparent in amounts of pentacyclic triterpenes were not apparent in the total amount of sterols extracted from the leaves (including surface wax) of the three varieties. Since S. bicolor varietal differences occurred only among the migrated hopanoids (found in mature leaves) it would appear that pentacyclic triterpenes, unlike sterols, have greater applicability as chemotaxonomic indices for intraspecific relationships in sorghum. 相似文献
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